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Plos One : Identification of Hypk-interacting Proteins Reveals Involvement of Hypk in Regulating Cell Growth, Cell Cycle, Unfolded Protein Response and Cell Death, Volume 7

By Beaudoin, Claude

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Book Id: WPLBN0003938958
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Reproduction Date: 2015

Title: Plos One : Identification of Hypk-interacting Proteins Reveals Involvement of Hypk in Regulating Cell Growth, Cell Cycle, Unfolded Protein Response and Cell Death, Volume 7  
Author: Beaudoin, Claude
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

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Beaudoin, C. (n.d.). Plos One : Identification of Hypk-interacting Proteins Reveals Involvement of Hypk in Regulating Cell Growth, Cell Cycle, Unfolded Protein Response and Cell Death, Volume 7. Retrieved from http://www.worldlibrary.in/


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Description : Huntingtin Yeast Two-Hybrid Protein K (HYPK) is an intrinsically unstructured huntingtin (HTT)-interacting protein with chaperone-like activity. To obtain more information about the function(s) of the protein, we identified 27 novel interacting partners of HYPK by pull-down assay coupled with mass spectrometry and, further, 9 proteins were identified by colocalization and co-immunoprecipitation (co-IP) assays. In neuronal cells, (EEF1A1 and HSPA1A), (HTT and LMNB2) and (TP53 and RELA) were identified in complex with HYPK in different experiments. Various Gene Ontology (GO) terms for biological processes, like protein folding (GO : 0006457), response to unfolded protein (GO : 0006986), cell cycle arrest (GO : 0007050), anti-apoptosis (GO : 0006916) and regulation of transcription (GO : 0006355) were significantly enriched with the HYPKinteracting proteins. Cell growth and the ability to refold heat-denatured reporter luciferase were decreased, but cytotoxicity was increased in neuronal cells where HYPK was knocked-down using HYPK antisense DNA construct. The proportion of cells in different phases of cell cycle was also altered in cells with reduced levels of HYPK. These results show that HYPK is involved in several biological processes, possibly through interaction with its partners.

 

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